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Sulfate reducing bacteria (SRB) | Sulfate reducing bacteria (SRB) |
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Sulfate reducing bacteria form one group of sulfate reducing prokaryotes. Main genus is Desulfovibrio. Desulfovibrio desulfuricans is often used to immobilize dissolved heavy metals as metallic sulfides. SRB are anaerobes that are sustained by organic nutrients. Generally they require a complete absence of oxygen and a highly reduced environment to function efficiently. Nonetheless, they circulate (probably in a resting state) in aerated waters, including those treated with chlorine and other oxidizers, until they find a "ideal" environment supporting their metabolism and multiplication.
SRB culture with a carbonate precipitate (The bacteria are about 6-8 µm long and 2 µm in diameter) SRB are usually lumped into two nutrient categories, those that can use lactate and those that cannot. The latter generally use acetate and are difficult to grow in the laboratory on any medium. Lactate, acetate, and other short chain fatty acids usable by SRB do not occur naturally in the environment. Therefore, these organisms depend on other organisms to produce such compounds. SRB reduce sulfate ion (SO4-2) to sulfide ion (S-2), which usually shows up as hydrogen sulfide, with the concomitant oxidation of a carbon source or, if iron is available, as black ferrous sulfide. In the absence of sulfate, some strains can function as fermenters and use organic compounds such as pyruvate to produce acetate, hydrogen, and carbon dioxide. Many SRB strains also contain hydrogenase enzymes, which allow them to consume hydrogen. Most common strains of SRB grow best at temperatures from 25° to 35°C. A few thermophilic strains capable of functioning efficiently at more than 60°C have been reported. The lignite, lignin, tannins, cellulose, starches and fatty acids found in many mud systems are carbon food sources for SRB. Where mud is stored, precautions should always be taken when handling or reconditioning water muds containing lignosulfonates, gypsum (sulfate sources) and starches, cellulose, xanthan gum and lignite (food sources). These muds can harbor SRB and can have high sulfide accumulations. Mud filtrate should be tested with the Garrett Gas Train to determine sulfide concentration in a stored mud, followed by treatments with caustic soda to raise pH and zinc-based scavengers to remove sulfides as ZnS. Before storage of mud, treatment with a bactericide can inhibit SRB growth. Also, circulating mud from time to time, with air entrainment, can retard development of anaerobic conditions. |
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